GetPrimers
GetPrimers

Advanced settings
Primer melting temperature (Tm):
Min Opt Max Max Tm difference
Primer size and Max Poly-X:
Min Opt Max Max Poly-X
PCR product size:
Primer GC content (%):
Min Max Min Max
Exon-save distances:
Primers-search distances:
5'-exon-save distance 3'-exon-save distance F-search distance R-search distance
Cross-exons max size (b.p.):
Max MAF:
Max Max
GRCh37
GRCh38

Quick settings